DNA 恒溫快速擴(kuò)增試劑盒（基礎(chǔ)型） DNA Isothermal Rapid Amplification Kit（Basic)

產(chǎn)品參數(shù)Product parameters：

注 Tips：

1. 高溫變性不能有效去除蛋白，可能對(duì)產(chǎn)物分析造成影響；High-temperature denaturation cannot effectively produce protein and may affect product analysis;

2. 市場(chǎng)上部分?jǐn)U增產(chǎn)物純化試劑盒不適用，可能造成假陰性結(jié)果。Some amplification product purification kits on the market are not suitable and may cause false negative results.

原理概述 Principle overview：

本試劑盒基于一種常溫恒溫核酸快速擴(kuò)增技術(shù)：在常溫恒溫下（一般為39ºC~42ºC），在輔助蛋白和單鏈結(jié)合蛋白的幫助下，重組酶和引物形成復(fù)合體；進(jìn)行同源搜索并結(jié)合目的同源域，此時(shí)在該同源位置形成D-loop區(qū)并開始進(jìn)行鏈交換；伴隨著重組酶從復(fù)合體上解離，聚合酶也結(jié)合到引物的3’末端，開始鏈的延伸。

This kit is based on a room temperature and constant temperature nucleic acid rapid amplification technology: at room temperature and constant temperature (generally 39ºC~42ºC), with the help of auxiliary proteins and single-chain binding proteins, the recombinase and primers form a complex; homology search is performed And combines with the target homologous domain, at this time, a D-loop region is formed at the homologous position and strand exchange begins; as the recombinase dissociates from the complex, the polymerase also binds to the 3' end of the primer, starting the chain exchange extend.

引物設(shè)計(jì) Primer design：

建議使用長(zhǎng)度在 30-35 bp 的引物，引物過短會(huì)影響擴(kuò)增速度和檢測(cè)靈敏度；引物設(shè)計(jì)避免形成二級(jí)結(jié)構(gòu)而影響擴(kuò)增；擴(kuò)增子長(zhǎng)度建議在 150-300 bp，通常不超過 500 bp。

It is recommended to use primers with a length of 30-35 bp. Primers that are too short will affect amplification speed and detection sensitivity; primers are designed to avoid the formation of secondary structures that affect amplification; the amplicon length is recommended to be 150-300 bp, usually no more than 500 bp.

由于試劑盒靈敏度非常高，在進(jìn)行反應(yīng)時(shí)請(qǐng)注意避免核酸污染，并設(shè)置空白對(duì)照；（圖片為凝膠電泳法顯示結(jié)果）

Since the sensitivity of the kit is very high, please be careful to avoid nucleic acid contamination during the reaction and set a blank control; (The picture shows the results of gel electrophoresis)

產(chǎn)品特點(diǎn)及優(yōu)勢(shì)Product features and advantages：

本試劑盒具有靈敏度高、特異性強(qiáng)、反應(yīng)時(shí)間短（僅需 30 min）等優(yōu)點(diǎn)；反應(yīng)組分為干粉狀態(tài)，操作簡(jiǎn)便，易于保存。使用金屬浴、水浴鍋等即可進(jìn)行反應(yīng)操作；無需購(gòu)買 PCR 儀等價(jià)格高昂的設(shè)備。

This kit has the advantages of high sensitivity, strong specificity, and short reaction time (only 30 minutes). The reaction components are in dry powder state, which is easy to operate and easy to store. Reaction operations can be performed using metal baths, water baths, etc.; there is no need to purchase expensive equipment such as PCR machines.

產(chǎn)品用途Application：

適用于實(shí)驗(yàn)室級(jí)別的DNA擴(kuò)增以及其他檢測(cè)用途的DNA擴(kuò)增使用。

Suitable for laboratory-grade DNA amplification and DNA amplification for other detection purposes.

操作步驟 Operation procedure and process：

實(shí)驗(yàn)操作相關(guān)視頻，請(qǐng)微信掃碼聯(lián)系安普未來生物客服獲取。

For videos related to experimental operations, please scan the QR code on WeChat to contact customer service.

產(chǎn)品線Product Line：

性能對(duì)比數(shù)據(jù)Performance comparison data：

Q：如何提高結(jié)果準(zhǔn)確度？

A：使用前請(qǐng)仔細(xì)閱讀說明書，嚴(yán)格按照操作步驟進(jìn)行操作；

Please read the instruction manual carefully before use and strictly follow the operating steps.

Q：如何降低實(shí)驗(yàn)室污染，避免出現(xiàn)假陽性？

A：樣品處理應(yīng)該嚴(yán)格按照生物安全規(guī)范操作，在進(jìn)行反應(yīng)時(shí)請(qǐng)注意避免核酸污染，并設(shè)置空白對(duì)照；

Sample processing should be done in strict accordance with biosafety regulations. Please be careful to avoid nucleic acid contamination when performing reactions and set up blank controls.

Q：不能一次性使用完所有試劑，應(yīng)該如何處理？

A：使用時(shí)請(qǐng)取出實(shí)驗(yàn)所需的凍干試劑數(shù)量，剩余部分請(qǐng)置于存儲(chǔ)條件下，注意蓋子密封好；

 When using, please take out the amount of freeze-dried reagent required for the experiment, and keep the remaining part under storage conditions. Make sure the lid is tightly sealed;

Q：試劑和組分不同批次是否可以混著用？

A：使用有效期內(nèi)試劑，且各組分不得與其他批號(hào)的相應(yīng)成分混用。

Use reagents within the validity period, and each component must not be mixed with corresponding components from other batches.